Jun-Jie (Gogo) Liu, Ph.D.
2007-2011，B.S., School of Life Sciences, Sichuan University
2011-2016，Ph.D., Peking University-Tsinghua University-NIBS Joint Ph.D. Program
2016-2018，Postdoctoral Fellow, MBIB Division, Lawrence Berkeley National Laboratory
2018-2020，Postdoctoral Fellow, Department of Molecular and Cell biology, UC Berkeley
2018-2020，Life Science Research Fund Fellow Sponsored by Pfizer
2020,10- Tenure-Track Assistant Professor, School of Life Sciences, Tsinghua University
Research Interest:
The Liu lab uses multidisciplinary approaches to study the following topics:
（1） Co-evolution of RNA and protein in RNP system with essential function across life domains.
（2） Identification, evolution study and application development of new CRISPR systems
（3） Functional and structural study of human disease related LncRNA and the development of new therapy method.
Selected Publications:
1. Deng P#, Tan SQ#, Yang QY, Fu L, Wu Y, Zhu HZ, Sun L, Bao Z, Lin Y, Zhang QC, Wang H, Wang J*, Liu JJ*. Structural RNA components supervise the sequential DNA cleavage in R2 retrotransposon. Cell. 2023 Jun 22;186(13):2865-2879.
2. Sun A#, Li CP#, Chen Z#, Zhang S#, Li DY, Yang Y, Li LQ, Zhao Y, Wang K, Li Z, Liu J, Liu S, Wang J*, Liu JJ*. The compact Casπ (Cas12l) 'bracelet' provides a unique structural platform for DNA manipulation. Cell Res. 2023 Mar;33(3):229-244.
3. Hu Z#, Sun A#, Yang J, Naz G, Sun G, Li Z, Liu JJ*, Zhang S*, Zhang X*. Regulation of the CRISPR-Cas12a system by methylation and demethylation of guide RNA. Chem Sci. 2023 May 9;14(22):5945-5955.
4. Li DY#, Li LQ#, Liu JJ*. Nucleases in gene-editing technologies: past and prologue. National Science Open. 2023 (in press)
5. Tsuchida CA#, Zhang S#, Doost MS#, Zhao Y#, Wang J, O'Brien E, Fang H, Li CP, Li D, Hai ZY, Chuck J, Brötzmann J, Vartoumian A, Burstein D, Chen XW, Nogales E, Doudna JA*, Liu JJ*. Chimeric CRISPR-CasX enzymes and guide RNAs for improved genome editing activity. Mol Cell. 2022 Mar 17;82(6):1199-1209.
6. Yang M, Sun R, Deng P, Yang Y, Wang W, Liu JJ*, Chen C*. Nonspecific interactions between SpCas9 and dsDNA sites located downstream of the PAM mediate facilitated diffusion to accelerate target search. Chem Sci. 2021 Aug 20;12(38):12776-12784.
7. Liu TY#, Liu JJ#, Aditham AJ, Nogales E, Doudna JA*. Target preference of Type III-A CRISPR-Cas complexes at the transcription bubble. Nat Commun. 2019 Jul 5;10(1):3001.
8. Liu JJ#, Orlova N#, Oakes BL#, Ma E, Spinner HB, Baney KLM, Chuck J, Tan D, Knott GJ, Harrington LB, Al-Shayeb B, Wagner A, Brötzmann J, Staahl BT, Taylor KL, Desmarais J, Nogales E*, Doudna JA*. Author Correction: CasX enzymes comprise a distinct family of RNA-guided genome editors. Nature. 2019 Apr;568(7752):E8-E10.
9. Jiang F#, Liu JJ#, Osuna BA#, Xu M, Berry JD, Rauch BJ, Nogales E, Bondy-Denomy J*, Doudna JA*. Temperature-Responsive Competitive Inhibition of CRISPR-Cas9. Mol Cell. 2019 Feb 7;73(3):601-610.
10. Wright AV#, Liu JJ#, Knott GJ, Doxzen KW, Nogales E, Doudna JA*. Structures of the CRISPR genome integration complex. Science. 2017 Sep 15;357(6356):1113-1118.
#: Co-first author; *: Corresponding author
Contact info:
email：junjiegogoliu@tsinghua.edu.cn
Tel：+86-010-62770270 (lab & office)
Address：A401, Bio-Medical Building, School of Life Sciences, Tsinghua University, Beijing, 100084, China
Lab Page：http://gogolab.life.tsinghua.edu.cn/