Fuchou Tang
Email: tangfuchou (at)pku(dot)edu(dot)cn；
Office: College of Life Sciences, Peking University, 100871
Research Area:
Stem cells have self-renewal ability and multi-lineage differentiation potential and are crucial for mammalian embryonic development and adult life. Dissecting the molecular mechanisms of stem cell regulation is important for understanding mammalian growth and development, and for its application of regeneration medicine for curing human diseases. Our lab focuses on deciphering the gene regulation network in human early embryos as well as the epigenetic regulation mechanisms of self-renewal ability and pluripotency of human pluripotent stem cells. Through this, we want to understand how the gene expression is regulated during human early embryonic development and how the global epigenetic reprogramming is achieved during the development of human primordial germ cells. We use single cell microRNA profiling assay, single cell RNA-Seq transcriptome analysis, and single cell DNA methylome sequencing technique we recently developed, as well as single cell genome sequencing, ChIP-Seq, micro-manipulation of mammalian early embryos to analyze the epigenetic regulation of the gene expression network in mammalian pluripotent stem cells and early embryonic cells.
Selected Publications:
1. Zhou F, Li X, Wang W, Zhu P, Zhou J, He W, Ding M, Xiong F, Zheng X, Li Z, Ni Y, Mu X, Wen L, Cheng T, Lan Y, Yuan W*, Tang Fuchou*, Liu B*. Tracing haematopoietic stem cell formation at single-cell resolution. Nature, 2016, doi: 10.1038/nature17997 (*: Co-corresponding authors)
2. Wen L*, Tang Fuchou*. Single-cell sequencing in stem cell biology. Genome Biology, 2016, 17: 71 (Review) (*: Co-corresponding authors).
3. Hou Y, Guo H, Cao C, Li X, Hu B, Zhu P, Wu X, Wen L, Tang Fuchou*, Huang Y*, Peng J*. Single-cell triple omics sequencing reveals genetic, epigenetic, and transcriptomic heterogeneity in hepatocellular carcinomas. Cell Research, 2016, 26: 304-319 (*: Co-corresponding authors).
4. Pan H, Guan D, Liu X, Li J, Wang L, Wu J, Zhou J, Zhang W, Ren R, Zhang W, Li Y, Yang J, Hao Y, Yuan T, Yuan G, Wang H, Ju Z, Mao Z, Li J, Qu J*, Tang Fuchou*, Liu GH*. SIRT6 safeguards human mesenchymal stem cells from oxidative stress by coactivating NRF2. Cell Research, 2016, 26: 190-205 (*: Co-corresponding authors).
5. Yan L, Guo H, Hu B, Li R, Yong J, Zhao Y, Zhi X, Fan X, Guo F, Wang X, Wang W, Wei Y, Wang Y, Wen L, Qiao J*, Tang Fuchou*. Epigenomic landscape of human fetal brain, heart, and liver. Journal of Biological Chemistry, 2016, 291: 4386-4398 (*: Co-corresponding authors).
6. Wen L*, Tang Fuchou*, How to catch rare cell types. Nature, 2015, 525: 197-198 (Preview) (*: Co-corresponding authors).
7. Guo F, Yan L, Guo H, Li L, Hu B, Zhao Y, Yong J, Hu Y, Wang X, Wei Y, Wang W, Li R, Yan J, Zhi X, Zhang Y, Jin H, Zhang W, Hou Y, Zhu P, Li J, Zhang L, Liu S, Ren Y, Zhu X, Wen L, Gao YQ, Tang Fuchou*, Qiao J*. The transcriptome and DNA methylome landscapes of human primordial germ cells. Cell, 2015, 161: 1437-1452 (*: Co-corresponding authors).
8. Zhang W, Li J, Suzuki K, Qu J, Wang P, Zhou J, Liu X, Ren R, Xu X, Ocampo A, Yuan T, Yang J, Li Y, Shi L, Guan D, Pan H, Duan S, Ding Z, Li M, Yi F, Bai R, Wang Y, Chen C, Yang F, Li X, Wang Z, Aizawa E, Goebl A, Soligalla RD, Reddy P, Esteban CR, Tang Fuchou*, Liu GH*, Belmonte JC*. A Werner syndrome stem cell model unveils heterochromatin alterations as a driver of human aging. Science, 2015, 348: 1160-1163 (*: Co-corresponding authors).
9. Wen L*, Tang Fuchou*, Charting a Map through the Cellular Reprogramming Landscape. Cell Stem Cell, 2015, 16: 215-216 (Preview) (*: Co-corresponding authors).
10. Wen L, Li J, Guo H, Liu X, Zheng S, Zhang D, Zhu W, Qu J, Guo L, Du D, Jin X, Zhang Y, Gao Y, Shen J, Ge H, Tang Fuchou*, Huang Y*, Peng J*. Genome-scale detection of hypermethylated CpG islands in circulating cell-free DNA of hepatocellular carcinoma patients. Cell Research, 2015, 25: 1250-1264 (*: Co-corresponding authors).
11. Yan L, Huang L, Xu L, Huang J, Ma F, Zhu X, Tang Y, Liu M, Lian Y, Liu P, Li R, Lu S, Tang Fuchou*, Qiao J*, Xie XS*. Live births after simultaneous avoidance of monogenic diseases and chromosome abnormality by next-generation sequencing with linkage analyses. Proc Natl Acad Sci U S A, 2015, 112: 15964-15969 (*: Co-corresponding authors).
12. Fan X, Zhang X, Wu X, Guo H, Hu Y, Tang Fuchou*, Huang Y*. Single-cell RNA-seq transcriptome analysis of linear and circular RNAs in mouse preimplantation embryos. Genome Biology, 2015, 16: 148 (*: Co-corresponding authors)
13. Guo H, Zhu P, Guo F, Li X, Wu X, Fan X, Wen L*, Tang Fuchou*. Profiling DNA methylome landscapes of mammalian cells with single-cell reduced-representation bisulfite sequencing. Nature Protocols, 2015, 10: 645-659 (*: Co-corresponding authors).
14. Duan S, Yuan G, Liu X, Ren R, Li J, Zhang W, Wu J, Xu X, Fu L, Li Y, Yang J, Zhang W, Bai R, Yi F, Suzuki K, Gao H, Esteban CR, Zhang C, Izpisua Belmonte JC, Chen Z, Wang X, Jiang T, Qu J*, Tang Fuchou*, Liu GH*. PTEN deficiency reprogrammes human neural stem cells towards a glioblastoma stem cell-like phenotype. Nature Communications, 2015, 6: 10068 (*: Co-corresponding authors).
15. Wu Y, Zhou H, Fan X, Zhang Y, Zhang M, Wang Y, Xie Z, Bai M, Yin Q, Liang D, Tang W, Liao J, Zhou C, Liu W, Zhu P, Guo H, Pan H, Wu C, Shi H, Wu L* , Tang Fuchou*, Li J,*. Correction of a Genetic Disease by CRISPR-Cas9-Mediated Gene Editing in Mouse Spermatogonial Stem Cells. Cell Research, 2015 , 25: 67-79 (*: Co-corresponding authors).
16. Shen J, Jiang D, Fu Y, Wu X, Guo H, Feng B, Pang Y, Streets AM, Tang Fuchou*, Huang Y*. H3K4me3 epigenomic landscape derived from ChIP-Seq of 1 000 mouse early embryonic cells. Cell Research, 2015, 25: 143-147 (*: Co-corresponding authors).
17. Guo F, Li X, Liang D, Li T, Zhu P, Guo H, Wu X, Wen L, Gu TP, Hu B, Walsh CP, Li J*, Tang Fuchou*, Xu GL*. Active and Passive Demethylation of Male and Female Pronuclear DNA in the Mammalian Zygote. Cell Stem Cell, 2014, 15: 447-458 (*: Co-corresponding authors).
18. Guo H, Zhu P, Yan L, Li R, Hu B, Lian Y, Yan J, Ren X, Lin S, Li J, Jin X, Shi X, Liu P, Wang X, Wang W, Wei Y, Li X, Guo F, Wu X, Fan X, Yong J, Wen L, Xie SX, Tang Fuchou*, Qiao J*. The DNA methylation landscape of human early embryos. Nature, 2014, 511: 606-610 (*: Co-corresponding authors).
19. Wen L, Tang Fuchou*, Reconstructing Complex Tissues from Single-Cell Analyses. Cell, 2014, 157: 771-773 (*: Corresponding author).
20. Wen L, Li X, Yan L, Tan Y, Li R, Zhao Y, Wang Y, Xie J, Zhang Y, Song C, Yu M, Liu X, Zhu P, Li X, Hou Y, Guo H, Wu X, He C*, Li R*, Tang Fuchou*, Qiao J*. Whole-genome analysis of 5-hydroxymethylcytosine and 5-methylcytosine at base resolution in the human brain. Genome Biology, 2014, 15: R49 (*: Co-corresponding authors).
21. Streets AM, Zhang X, Cao C, Pang Y, Wu X, Xiong L, Yang L, Fu Y, Zhao L*, Tang Fuchou*, Huang Y*. Microfluidic single-cell whole-transcriptome sequencing. Proc Natl Acad Sci U S A, 2014, 111: 7048-7053 (*: Co-corresponding authors).
22. Hou Y, Fan W, Yan L, Li R, Lian Y, Huang J, Li J, Xu L, Tang Fuchou*, Xie XS*, Qiao J*. Genome Analyses of Single Human Oocytes. Cell, 2013, 155: 1492-1506 (*: Co-corresponding authors).
23. Guo H, Zhu P, Wu X, Li X, Wen L, Tang Fuchou*. Single-cell methylome landscapes of mouse embryonic stem cells and early embryos analyzed using reduced representation bisulfite sequencing. Genome Research, 2013, 23: 2126-2135 (*: Corresponding author).
24. Yan L, Yang M, Guo H, Yang L, Wu J, Li R, Liu P, Lian Y, Zheng X, Yan J, Huang J, Li M, Wu X, Wen L, Lao K, Li R*, Qiao J*, Tang Fuchou*. Single-cell RNA-Seq profiling of human preimplantation embryos and embryonic stem cells. Nature Structural & Molecular Biology, 2013, 20: 1131-1139 (*: Co-corresponding authors).
25. Gan H, Wen L, Liao S, Lin X, Ma T, Liu J, Song CX, Wang M, He C, Han C*, Tang Fuchou*. Dynamics of 5-hydroxymethylcytosine during mouse spermatogenesis. Nature Communications, 2013, 4: 1995 (*: Co-corresponding authors).
26. Tang F*. Small RNAs in mammalian germline: tiny for immortal. Differentiation, 2010, 79: 141-146 (Review) (*: Corresponding author)